A New Fluorometric Method for the Estimation or Detection of Total and Fractionated Alkaline Phosphatase
نویسندگان
چکیده
منابع مشابه
A new fluorometric method for the estimation or detection of total and fractionated alkaline phosphatase.
A highly sensitivefluorometric method for the assayof total alkaline phosphataseand the detection of its components is described. The commercially available substrate naphthyl AS-MX phosphate, combined in 1 M 2-amino-2-methyl-1-propanol buffer, pH 9.8, is cleaved to the highly fluorescent naphthol AS-MX. Fluorometry requires filters passing405 m primary and 505 mi. secondary.As little as 20 1d....
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15 صفحه اولA new fluorometric turn-on assay for alkaline phosphatase and inhibitor screening based on aggregation and deaggregation of tetraphenylethylene molecules.
Based on the consideration that compound 1 with a -PO3H2 group can be hydrolyzed into compound 3 which shows low solubility in aqueous solutions and thus aggregation can occur easily, a new fluorescence turn-on assay has been constructed for alkaline phosphatase (ALP) with compound 1. ALP at concentrations as low as 18 mU mL(-1) can be assayed with compound 1. Moreover, compound 1 has been succ...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1969
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/15.2.108